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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in many tumors, which ends up in chromosome instability, and thus may perhaps add to tumorigenesis. This protein was identified to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial role in endorsing cell-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, happen to be described. Two added splice variants were being described but comprehensive nucleotide sequence information just isn't nevertheless available.
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Blocking peptides are peptides that bind particularly to your focus on antibody and block antibody binding. These peptide commonly is made up of the epitope recognized via the antibody.
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This guidebook outlines more attainable optimization of mobile and immunoassay parameters to be sure the absolute best effects are...
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This manual outlines even more attainable optimization of cellular and immunoassay parameters to be sure the very best success are...
Antibodies bound to the blocking peptide not bind into the epitope on the goal protein. By comparing the staining through the blocked antibody compared to the antibody on your own, one can see which staining is certain.
Goal Info Cyclin E belongs towards the highly conserved cyclin family, whose users are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add into the temporal coordination of every mitotic party. Cyclin E varieties a posh with and functions like a regulatory subunit of CDK2, whose exercise is necessary for mobile cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is particularly degraded as cells development as a result of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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